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涓村簥搴旂敤
(涓€)绮惧瓙DNA纰庣墖妫€娴嬮」鐩?/strong>
绮惧瓙鏌撹壊璐ㄧ粨鏋勭殑瀹屾暣鎬у浜庣簿纭紶閫掗仐浼犵墿璐ㄨ嚦鍏抽噸瑕併€傜簿瀛怐NA鎹熶激鑳藉褰卞搷绮惧瓙鐨勫彈绮捐兘鍔涖€佸彈绮惧嵉鐨勫垎瑁傘€佽儦鑳庣殑鍙戣偛浠ュ強瀛愪唬鍋ュ悍銆傜簿瀛愭煋鑹茶川缁撴瀯鍒嗘瀽(SCSA)鏄娴嬬簿瀛怐NA瀹屾暣鎬х殑涓€绉嶆柟娉曘€傜爺绌舵樉绀篋FI涓嶱R銆佺簿瀛愭祿搴︺€佺簿瀛愭甯稿舰鎬佺巼銆佺簿瀛愬瓨娲荤巼鍧囧憟璐熺浉鍏虫€с€傛彁绀篋FI涓庣簿瀛愭椿鍔涖€佸舰鎬佺瓑瀛樺湪涓€瀹氱殑鐩稿叧鎬э紝PR銆佺簿瀛愭祿搴︺€佹甯稿舰鎬佺巼銆佸瓨娲荤巼浼氶殢鐫€DFI鐨勫楂樿€岄檷浣庛€備絾骞朵笉鑳藉畬鍏ㄨ鏄庣簿瀛愬舰鎬佹甯哥巼銆佸瓨娲荤巼闄嶄綆锛屽氨浼氭崯浼ょ簿瀛怐NA锛屼絾鍙楁崯鐨勭簿瀛怐NA浼氬绮惧瓙鐨勫彈绮捐兘鍔涢€犳垚褰卞搷锛屼粠鑰屽鑳氳儙鍙戣偛璧峰埌鎶戝埗浣滅敤銆傜患涓婃墍杩帮紝DFI澧為珮涓庝笉鑲茬棁鐨勫彂鐢熻仈绯诲瘑鍒囷紝DFI涓嶱R銆佺簿瀛愭祿搴︺€佺簿瀛愭甯稿舰鎬佺巼銆佺簿瀛愬瓨娲荤巼鍧囧憟璐熺浉鍏虫€э紝鍦ㄤ笉鑲茬棁鎮ｈ€呯梾鎯呰瘎浼颁腑鍏锋湁閲嶈鐨勫弬鑰冧环鍊硷紝鍊煎緱鍊熼壌[5]銆侟/p>
(浜?绮惧瓙绾跨矑浣撴娴嬮」鐩?/strong>
MMP鍙弽鏄犵簿瀛愯川閲忎笌绾跨矑浣撳姛鑳戒箣闂寸殑瀵嗗垏鍏崇郴銆傛棭鍦?982骞达紝Evenson绛夊氨鍙戠幇寮辩簿瀛愮棁鎮ｈ€呯簿瀛怣MP姘村钩杈冧綆銆傜爺绌惰繘涓€姝ヨ瘉瀹濵MP涓庣簿瀛愭椿鍔涖€佹甯稿舰鎬佷箣闂村瓨鍦ㄧ浉鍏虫€?銆傛潹棰栫瓑鎶ラ亾寮辩簿瀛愮粍MMP浣庝簬瀵圭収缁勶紝涓擬MP涓庣簿瀛愭椿鍔ㄧ巼銆佸墠鍚戣繍鍔ㄧ簿瀛愮櫨鍒嗙巼鍙婃甯稿舰鎬佺簿瀛愮櫨鍒嗙巼鍛堟鐩稿叧銆俍hag绛夋姤閬撴甯告按骞崇殑绮惧瓙MMP鏄淮鎸佺簿瀛愰《浣撻叾娲绘€у拰鏌撹壊璐ㄥ畬鏁存€х殑鐢熺悊鍩虹锛孧MP浣庣殑绮惧瓙涓嶆槗杩涜椤朵綋鍙嶅簲銆傚洜姝わ紝鍥藉瀛﹁€呭缓璁涓嶆槑鍘熷洜涓嶈偛澶涓旂敺鏂圭簿瀛怣MP姘村钩杈冧綆鏃讹紝閲囩敤鍗电粏鑳炶川鍐呭崟绮惧瓙娉ㄥ皠鎶€鏈?ICSI)娌荤枟銆侻MP鍜岀簿瀛怐NA纰庣墖鎸囨暟(DFI)鑱斿悎棰勬祴濂虫€ч厤鍋惰嚜鐒跺彈瀛曠殑鑳藉姏浼樹簬绮炬恫甯歌鍙傛暟锛屾槸涓村簥璇勪及鐗瑰彂鎬х敺鎬т笉鑲茬殑閲嶈鎸囨爣[6]銆侟/p>
(涓?绮惧瓙椤朵綋妫€娴嬮」鐩?/strong>
绮惧瓙椤朵綋閰舵椿鎬у彲浣滀负绮惧瓙鍙戠敓鍜岀簿瀛愯川閲忕殑鍒嗗瓙鏍囪锛屽苟涓庣敺鎬х殑鐢熻偛娼滃姏瀵嗗垏鐩稿叧锛屽彲浠ヤ笌鍏朵粬鐢熺墿鏍囧織鐗╄仈鍚堟娴嬶紝濡傜簿娑插弬鏁板拰绮惧瓙DNA纰庣墖鍖栨寚鏁帮紝瀵规彁楂樼敺鎬т笉鑲茬棁鐨勮瘖鏂晱鎰熸€у強棰勬祴鑷劧濡婂鎴愬姛鐨勮兘鍔涜捣鐫€鑷冲叧閲嶈鐨勪綔鐢?span style="color:hsl(212,80%,42%);">[7]銆侟/p>
(鍥?绮惧瓙娲绘€ф哀绫荤墿璐ㄦ娴嬮」鐩?/strong>
瀹嬫檽搴嗙瓑[8]鐮旂┒琛ㄦ槑锛岀簿瀛怐FI鍊间笌绮惧瓙娲绘€ф哀(ROS)姘村钩瀛樺湪姝ｇ浉鍏虫€э紝涔熸彁绀虹簿瀛怐NA鎹熶激鍙兘鏄敱浜庣簿娑蹭腑姘у寲搴旀縺鍙嶅簲閫犳垚鐨勩€傚紓甯稿舰鎬佺簿瀛愬彲鑳芥槸浜х敓姘ц嚜鐢卞熀鐨勯噸瑕佹潵婧愶紝鑰岄珮姘村钩绮惧瓙ROS涓庣簿瀛怐NA纰庣墖澧炲鍙兘鏈夊叧锛屽畠浼氬姞閫熺敓娈栫粏鑳炵殑璋冧骸銆佸鑷村彈绮剧巼闄嶄綆绛変竴绯诲垪涓嶈壇鍚庢灉銆侟/p>
閫傜敤绉戝
鐢风銆佺敓娈栧尰瀛︾銆佹硨灏垮绉戙€佸浜х銆佷笉瀛曚笉鑲茬銆佹楠岀
鍙傝€冩枃鐚?/h3>
[1] 鍒樼鐞 and 钁ｅ缓鍐? 鐢锋€т笉鑲茬棁鎮ｈ€呯簿瀛怐NA纰庣墖鎸囨暟妫€娴嬫剰涔? 瀹炵敤鍖绘妧鏉傚織, 2024. 31(04): p. 237-240.
[2] 鏉庣孩鏋? 鍌呭箍娉? and 鍚曡堪褰? 绮惧瓙绾跨矑浣撳姛鑳芥娴嬩笌鐢锋€т笉鑲茬浉鍏虫€х殑鐮旂┒杩涘睍. 涓村簥妫€楠屾潅蹇? 2021. 39(04): p. 287-290.
[3] 閭卞皬榫? et al., 绮惧瓙椤朵綋閰舵椿鎬у湪鐢锋€х敓娈栦腑鐨勪环鍊肩爺绌惰繘灞? 娌冲寳鍖昏嵂, 2024. 46(06): p. 914-918.
[4] 瀹嬫檽搴? et al., 绮惧瓙娲绘€ф哀绫荤墿璐ㄤ笌鍏跺舰鎬佸強DNA纰庣墖鐨勭浉鍏虫€? 涓浗璁″垝鐢熻偛瀛︽潅蹇? 2022. 30(02): p. 387-390.
鎸囧崡鍏辫瘑
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1 |
銆婄簿瀛怐NA纰庣墖妫€娴嬬殑涓村簥涓撳鍏辫瘑銆婞/p> |
绮惧瓙DNA纰庣墖(sperm DNA fragmentation,SDF)鏄竴椤规柊鐨勫弽鏄犵簿瀛愬姛鑳界殑妫€娴嬫寚鏍囥€係DF澧為珮甯镐即鍙戝皯銆佸急绮惧瓙鐥囷紝涔熷彲鍙戠敓鍦ㄧ簿娑插父瑙勬寚鏍囨甯哥殑鐢锋€т笉鑲茬棁鎮ｈ€呫€係DF鐨勫彂鐢熸満鍒跺鏉傘€傚緢澶氭枃鐚姤閬揝DF鍗囬珮涓庤嚜鐒跺濞犲拰杈呭姪鐢熸畺鎶€鏈粨灞€瀛樺湪璐熺浉鍏炽€傚洜姝わ紝杩戝勾鏉DF妫€娴嬪凡骞挎硾搴旂敤浜庣敺鎬т笉鑲茬殑璇婃柇鍜屾不鐤椼€侟/p> |
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銆婄簿瀛愮鐗囧疄楠屽妫€娴嬩笌涓村簥搴旂敤涓撳鍏辫瘑——鍩轰簬娴佸紡缁嗚優绮惧瓙鏌撹壊璐ㄧ粨鏋勫垎鏋愩€婞/p> |
鍦ㄨ繃鍘荤殑鍑犲崄骞撮噷锛岀簿瀛怐NA纰庣墖(SDF)妫€娴嬫槸涓€椤圭粡鍙椾綇鏃堕棿鑰冮獙鐨勭簿瀛愬姛鑳界爺绌舵妧鏈紝鍦ㄥ叏涓栫晫鐨勭敺绉戝疄楠屽涓棩鐩婃櫘鍙娿€傚悇绉嶄綋澶栧拰浣撳唴鐮旂┒閮芥敮鎸佺簿瀛怐NA瀹屾暣鎬у湪鍙楃簿銆佹棭鏈熻儦鑳庡彂鑲层€佹鍏ュ拰濡婂涓殑閲嶈鎬с€傚浜庢帴鍙楄緟鍔╃敓娈栨妧鏈殑鎮ｈ€咃紝SDF宸茬粡鎴愪负涓€绉嶆柊鐨勯闄╁垎灞傜敓鐗╂爣蹇楃墿銆侟/strong> |
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